Patrick Kellish
University of Florida, FL·Microbiology & Immunology
Much of modern cancer research depends on being able to identify and separate single cells and to watch what happens inside them. Two technologies do most of this work: flow cytometry, which streams cells past lasers one at a time to read which marker proteins each cell carries and can physically sort cells into tubes, and confocal microscopy, which uses focused laser light to build sharp optical slices of living cells and tissue.
- Fields
Microbiology & Immunology
Medicine & Clinical Research
Biology
- Topics
flow cytometry
cell sorting
confocal microscopy
- Grants
- 1 known grant, through May 2027
What they work on
- Provide multi-parameter flow cytometry and cell sorting, which reads marker proteins on individual cells and separates populations, to cancer center investigators as a cost-recovered shared service.
- Deliver confocal microscopy for live-cell imaging and assessment of tissue and cell morphology, plus analysis of small particles and exosomes, the membrane vesicles cells secrete.
- Perform flow-based immunophenotyping of tumor and immune cells to support first-in-human RNA therapy clinical trials, with a PhD-level immunologist recruited for this work.
- Train students, postdoctoral fellows and technical staff on cytometry and imaging instruments, and expand advanced instruction in applying biostatistics to cytometric data with the biostatistics shared resource.
- Expand capabilities by acquiring a high-parameter cell sorter and increasing super high-resolution microscopy and single-cell analysis services.
More about the research
Both require expensive instruments and trained operators, which is why they are run as shared facilities rather than in each lab.
Why it matters
Shared instrumentation lets cancer researchers measure immune and tumor cell populations that individual labs could not afford to study alone, including immune monitoring for early-phase RNA therapy trials in patients. The facility's training of hundreds of students and fellows also builds practical single-cell measurement skills that transfer across biomedical fields.
Known grants
From NIH and NSF research grants. Labs often have other funding we can't see.
Closest labs
Zuhair Ballas
University of Iowa, IA·Medicine & Clinical Research
Provide cancer researchers with high-speed cell sorting, multi-parameter analysis and purification of specific cell subsets for basic and translational studies at the Holden Comprehensive Cancer Center.
Karen Creswell
Georgetown University, DC·Microbiology & Immunology
Provide flow cytometry analysis and cell sorting to cancer center members at two sites, sorting cells by transfection markers, stem cell panels and surface or internal markers for functional and molecular follow-up studies.
Zoran Galic
University of California-Los Angeles, CA·Medicine & Clinical Research
Provide cancer center investigators with flow cytometry, mass cytometry, imaging cytometry and cell sorting services using instruments such as the Helios mass cytometer, ImageStream imaging cytometer and Aria high-speed sorters.
The school
University of Florida: 65 labs on PI Pages, $1.3B a year on research, 795 research doctorates a year.
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