Karen Creswell
Georgetown University, DC·Microbiology & Immunology
Many questions in cancer and immune research depend on measuring the individual cells in a sample. Researchers need to know which cell types are present, what proteins each carries, and whether cells are dividing, dying or under stress.
- Fields
Microbiology & Immunology
Medicine & Clinical Research
Biology
- Topics
flow cytometry
cell sorting
mass cytometry
- Grants
- 1 known grant, through Apr 2029
What they work on
- Provide flow cytometry analysis and cell sorting to cancer center members at two sites, sorting cells by transfection markers, stem cell panels and surface or internal markers for functional and molecular follow-up studies.
- Offer functional assays for cell cycle, apoptosis and oxidative stress, and immunophenotyping of mouse and human tissues and tumor-infiltrating lymphocytes, using the 16-color LSRFortessa and 28-color FACSymphony A3 analyzers.
- Expand analysis to more than 40 simultaneous markers by adding a Standard BioTools CyTOF XT mass cytometry system for cancer and immune studies.
More about the research
Often they also need to separate one cell population out for further experiments. Flow cytometry does this by passing cells one at a time past lasers and reading the fluorescent labels attached to them. Cell sorting adds a step that physically separates the labeled cells.
Why it matters
By giving cancer researchers on-site access to cell analysis, cell sorting and expanded high-marker cytometry, the facility supports studies of tumor biology and of how the immune system interacts with cancer. It has contributed to clinical trials and published studies, and its services could help researchers find immune cell patterns relevant to cancer patients.
Known grants
From NIH and NSF research grants. Labs often have other funding we can't see.
Closest labs
Zoran Galic
University of California-Los Angeles, CA·Medicine & Clinical Research
Provide cancer center investigators with flow cytometry, mass cytometry, imaging cytometry and cell sorting services using instruments such as the Helios mass cytometer, ImageStream imaging cytometer and Aria high-speed sorters.
Zuhair Ballas
University of Iowa, IA·Medicine & Clinical Research
Provide cancer researchers with high-speed cell sorting, multi-parameter analysis and purification of specific cell subsets for basic and translational studies at the Holden Comprehensive Cancer Center.
Larry Harshyne
Thomas Jefferson University, PA·Microbiology & Immunology
Provide high-quality, affordable analysis and sorting of cells, biological particles and extracellular vesicles using fluorescence-activated flow cytometers for cancer center researchers.
The school
Georgetown University: 14 labs on PI Pages, $377M a year on research, 206 research doctorates a year.
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