Arthur Salomon
University of California-San Francisco, CA·Biochemistry
Cells respond to signals from their surroundings through networks of proteins that pass messages to one another, often by adding phosphate groups (phosphorylation) or by binding partner proteins. Errors in these networks can cause disease, and knowing how they are wired can point to ways to treat it.
- Fields
Biochemistry
Microbiology & Immunology
Chemistry
- Topics
proteomics
mass spectrometry
T cell receptor signaling
- Grants
- 1 known grant, through Apr 2027
What they work on
- Identify the binding partners of signaling proteins in T cell lines and primary mouse T cells, using TurboID, a labeling enzyme that tags nearby proteins in living cells, followed by mass spectrometry.
- Map phosphorylation sites across T cell receptor signaling networks as deeply as possible, using a Src SH2 domain Superbinder, an engineered protein that captures phosphorylated sites, together with TMT BOOST labeling for higher sensitivity.
- Measure relative changes in protein levels and post-translational modifications across T cell activation states with quantitative LC/MS, a method that separates proteins and weighs their fragments.
- Apply statistical and computational tools to proteomic datasets to analyze them rigorously and predict new signaling pathways for the program's investigators to test.
More about the research
These networks are large and act within seconds, so they are hard to map one mutant at a time.
Why it matters
Mapping how T cell receptor signals are wired could reveal how faults in these networks lead to disease. That knowledge could suggest new points where drugs might intervene. Better tools for measuring phosphorylation and protein partners in immune cells give the supported investigators broad datasets for building and testing hypotheses about immune signaling.
Known grants
From NIH and NSF research grants. Labs often have other funding we can't see.
Closest labs
Vsevolod V. Gurevich
Vanderbilt University, TN·Biochemistry
Determine how arrestin-1 selectively binds light-activated, phosphorylated rhodopsin over its other forms, using direct binding assays with phosphorylated and unphosphorylated, light-activated and dark rhodopsin.
Adam Courtney
University of Michigan, MI·Microbiology & Immunology
Determine how negative feedback loops, in which activated kinases (enzymes that add phosphate tags) dampen their own pathway, shape distinct T cell receptor signaling outcomes, using chemical tools and immunological assays.
Ljiljana Pasa-Tolic
University of Arizona, AZ·Biochemistry
Refine a liquid chromatography–mass spectrometry (LC-MS) proteomics platform for sensitive, high-throughput protein profiling of single spiral ganglion neurons, which relay sound from the inner ear.
The school
University of California-San Francisco: 89 labs on PI Pages, $2.1B a year on research, 164 research doctorates a year.
Reach out
A start for your email. Fill in the brackets.