PIPages
Labs

Caitlyn Butler

University of Massachusetts, MA·Engineering

Treated wastewater still carries nitrogen and phosphorus, and when those nutrients reach rivers, lakes and estuaries they feed algal blooms and degrade water quality. Conventional treatment steps such as activated sludge, aerated lagoons and membrane bioreactors often cannot meet increasingly strict discharge limits, and the advanced options that can, like chemical precipitation or enclosed photobioreactors, are expensive and energy-hungry.

Fields

Engineering

Earth & Environmental Science

Microbiology & Immunology

Topics

oxygenic photogranules

wastewater treatment

nutrient removal

Grants
1 known grant, through Jul 2028

What they work on

  1. Demonstrate that oxygenic photogranules, dense clusters of algae, cyanobacteria and bacteria, stay intact and grow stably in nutrient-poor secondary effluent by measuring granule formation, size distribution, density, porosity and shear resistance.
  2. Assess microbial physiological responses to low-nutrient stress using extracellular polymeric substance profiling, enzyme activity assays, and RT-qPCR of genes for ammonia oxidation, denitrification and phosphorus uptake.
  3. Optimize sequencing batch reactor operation, including light intensity, mixing pattern, hydraulic retention time, solids retention time and shear stress, to maximize nitrogen and phosphorus removal rates.
  4. Deliver a scalable, low-energy post-secondary treatment process and a mechanistic account of how photogranules remove nutrients when nitrogen and phosphorus are limited.

More about the research

This project tests a low-energy alternative built on oxygenic photogranules (OPGs): dense, self-assembling clusters of cyanobacteria, algae and other bacteria that use sunlight to pull nutrients out of water. Because the granules are compact, they settle on their own, so no mechanical or chemical separation step is needed, and they can run in simple tanks with modest light and mixing.

Why it matters

Nutrient pollution from municipal wastewater drives eutrophication and harmful algal blooms in U.S. surface waters, yet the advanced treatment needed to meet strict discharge limits is costly for towns to build and run. A photogranule system that settles on its own and needs little energy, light or chemical addition could lower treatment costs for communities and household water bills while protecting sensitive watersheds.

Known grants

From NIH and NSF research grants. Labs often have other funding we can't see.

Exploring Oxygenic Photogranules (OPGs) for Enhanced Nutrient Removal in Secondary Effluent Wastewater

NSF · Aug 2025 – Jul 2028

Closest labs

Ian Bradley

University at Buffalo, NY·Engineering·Early-career

Identify the environmental and operating factors that improve total nitrogen and phosphorus removal in algae-based wastewater treatment, using lab-scale systems that grow algae on wastewater.

James Moberly

University of Idaho, ID·Engineering

Develop an encapsulated biological material in which phosphorus accumulating organisms, microbes that store large amounts of phosphorus, are immobilized within a polymer structure for use in wastewater treatment.

Sarah Preheim

Johns Hopkins University, MD·Earth & Environmental Science

Test how the abundance of denitrification genes, measured by quantitative PCR, relates to denitrification rates in controlled bioreactor experiments, and whether disturbance frequency changes that relationship.

The school

University of Massachusetts: 28 labs on PI Pages, $282M a year on research, 337 research doctorates a year.

More about University of Massachusetts

Reach out

A start for your email. Fill in the brackets.